BORG Presentations

 

(2017201620152014, 201320122011201020092008, 2007, 2006, 2005, 2004, 2003, 2002)

D.L. Pham and M.G. Nichols. Development and characterization of phasor-based analysis for FLIM to evaluate metabolic and epigenetic impacts of HER2 inhibition on Squamous Cell Carcinoma (SCC) cultures, NE-INBRE 15th Annual Conference, August 7, 2017, Nebraska City, NE

L. Aumen, J. List, M.G. Nichols, A. Csordas. Operator Exposure Distribution During Inverventonal Fluoroscopy Proceedures. Annual Meeting of the American Association of Physicists in Medicine, July 30, 2017, Denver, CO

D.L. Pham, K.D. Sotelo, M.F. Gabriel and Michael G. Nichols Applying Phasor FLIM to assess the metabolic impact of ultraviolet exposure and HER2 inhibition  in cultured Squamous Cell Carcinoma and in vivo skin. 2017 Central Region IDeA Meeting June 8, 2017. Sioux Falls, SD

S.A. Khan, M.G. Nichols, T. Miyamoto, D. R. Cerutis, Localization of the Platelet-Activating Factor Receptor on the Intact Human Periodontal Ligament and Label-free Imaging of Collagen Architecture. UNC 2017 Perio Expo, May 5 2017, Chapel Hill, NC.

M.G. Nichols, R. Das, M.F. Gabriel, C.R. Miller, A.M. Mills, D.M. Myers, D.L. Pham, I.N. Pociupany, K. D. Sotelo, K. E. Troup Metabolic imaging of Squamous cell carcinoma cells and in vivo skin by phasor FLIM. Biophysical Society 61st Annual Meeting, February 12, 2017, New Orleans, LA (Undergraduate Symposium)

MF A. Gabriel, K. D. Sotelo, C.R. Miller, A.M. Mills, D.L. Pham, I.N. Pociupany, D.M. Myers, M.G. Nichols. Assessing the impact of ultraviolet exposure on cellular metabolism of cultured squamous cells and in vivo skin using phasor FLIM analysis, WoPhyS 8th annual meeting, October 27, 2016, Lincoln, NE

D.M. Myers, C.R. Miller, D.L. Pham, I.N. Pociupany, M.G. Nichols. Phasor‐based analysis of fluorescence lifetime images reveals metabolic changes in Squamous Cell Carcinoma cultures, WoPhyS 8th annual meeting, October 27, 2016, Lincoln, NE

M.G. Nichols, M.F. Gabriel, C.R. Miller, A.M. Mills, D.M. Myers,  D.L. Pham, I.N. Pociupany, K.D.  Sotelo, K.E. Troup, Progress toward metabolic imaging of disease progression in skin cancer by FLIM, NE-INBRE 14th Annual Conference, August 7, 2016, Nebraska City, NE

S. Prathivadhi, C. Taylor, M. Nichols, A. Ekpenyong, Effects of chemotherapy-induced alterations in cell mechanical proterties on cancer metastasis, 126th annual meeting of the Nebraska Academy of Sciences, April 22, 2016, Lincoln, NE

S. Prathivadhi, J. Ning C. Taylor, M.G. Nichols, and A. Ekpenyong Effects of chemotherapy-induced alterations in cell mechanical properties on cancer metastasis. American Physical Society March Meeting, March 14-18, 2016, Baltimore, MD

S. Prathivadhi, C. Taylor, J. Ning, M.G. Nichols, A. Ekpenyong. Cell mechanical properties and cancer metastasis: effects of cancer drugs and radiotherapy. Biophysical Society 60th Annual Meeting, March 3, 2016, Los Angeles, CA.

D. Desa, E. Riley, M.G. Nichols, H. Jensen Smith. Ototoxic Aminoglycosides inhibit NADH metabolism and increase reactive oxygen species formation in Cochlear cells. Biophysical Society 60th Annual Meeting, March 2, 2016, Los Angeles, CA. 

E. Effa, A. Ikpeme, M. Inyama, B. Agu, T. Ekpenyong, A. Otu, N. Ani,W. Egbe, E.A. Tom, S. Prathivadhi, M.G. Nichols, S. Kosoko-Lasaki, A. Ekpenyong. Mobile Clinics Deployed in Rural Nigeria for Free Patient Care and Clinical Research. Global Health Conference Midwest, February 5, 2016, Omaha, NE.

C. Taylor , S. Prathivadhi, J. Ning, M.G. Nichols, A. Ekpenyong. Microfluidic devices for biomechanical assessment of metastatic effects of cancer drugs. Global Health Conference Midwest, February 5, 2016, Omaha, NE.

2015

C. Miller. Applying nonlinear optics to monitor changes in cellular metabolism with the onset and progression of disease, WoPhyS 7th annual meeting , October  15, 2015, Lincoln, NE

D. Desa, M. G. Nichols and H. Jensen Smith. Ototoxic aminoglycosides increase reactive oxygen species and decrease endogenous antioxidant levels in cochlear cells, WoPhyS 7th annual meeting , October  15, 2015, Lincoln, NE

D. L. Pham, K. D. Sotelo, M. F. Gabriel, A. M. Mills, I. N. Pociupany, K. E. Troup, D. M. Myers, M. G. Nichols Assessing physiological and environmental factors that affect optical profiling by lifetime imaging of endogenous cellular fluorescence, WoPhyS 7th annual meeting , October  15, 2015, Lincoln, NE

C. Miller.  Scavenging of Reactive Oxygen Species for Prevention of Ultraviolet Radiation Induced Skin Damage. 13th Annual Meeting of the Nebraska IDeA Network for Biomedical Research Excellence Conference in Lincoln, Nebraska. August 3, 2015. (Best Oral Presentation Award)

D. Pham and M.G. Nichols.  Monitoring the regulation of cellular metabolic state and epigenetic activities in cancerous cells by NADH and flavoprotein fluorescent lifetime imaging microscopy (FLIM). Creighton University St. Albert's Day/ University Research Day, April 14, 2015, Omaha, NE

K. Arnold, L. Higuchi, K. Kramer, M.G. Nichols, H. Jensen Smith.  Differences in mitochondrial metabolism in regenerative and non-regenerative sensory epithelia. Creighton University St. Albert's Day/ University Research Day, April 14, 2015, Omaha, NE

C. Altfillish, K. Arnold, L. Higuchi, K. Kramer, M.G. Nichols, H. Jensen Smith.  Differences in aminoglycoside entry and mitochondrial metabolism in regenerative and non-regenerative sensory epithelia. Creighton University St. Albert's Day/ University Research Day, April 14, 2015, Omaha, NE

C. Miller and M.G. Nichols.  Metabolic profiling of Squamous Cell Carcinoma through time and wavelength-resolved multiphoton microscopy. Creighton University St. Albert's Day/ University Research Day, April 14, 2015, Omaha, NE

C. Miller and M.G. Nichols. Optical metabolic profiling of squamous cell carcinoma. 125th annual meeting of the Nebraska Academy of Sciences, April 17, 2015, Lincoln, NE

D. Desa, M.G. Nichols, H. Jensen Smith. Ototoxic aminoglycosides increase reactive oxygen species while decreasing NADH reduction capacity at Complex 1. 125th annual meeting of the Nebraska Academy of Sciences, April 17, 2015, Lincoln, NE

D. Desa. Ototoxic aminoglycosides increase reactive oxygen species while decreasing NADH reduction capacity at Complex 1. 11th annual Honors Day presentation, April 15, 2015, Creighton University, Omaha, NE

C. R. Miller, M.G Nichols. Metabolic profiling of the skin to monitor the onset and progression of squamous cell carcinoma through time- and wavelength-resolved fluorescence lifetime imaging. Biophysical Society 59th Annual Meeting, February 10, 2015, Baltimore, CA.

D. Desa, M.G. Nichols, H. Jensen Smith. The Role of Complex I in Mitochondrial Reactive Oxygen Species Formation in Cochlear Sensory and Supporting Cells During Aminoglycoside Exposure. Biophysical Society 59th Annual Meeting, February 11, 2015, Baltimore, CA.

 

2014

C. Miller and M.G. Nichols. Shining a light on cellular metabolism of skin cancer through time- and wavelength-resolved multiphoton microscopy. Nebraska Neuroscience Symposium, September 24, 2014, Omaha, NE

D. Desa and H. Jensen Smith. Evaluating mitochondrial superoxide formation in cochlear cells during antibiotic exposure. Nebraska Neuroscience Symposium, September 24, 2014, Omaha, NE

A.C. Jenzer, R.C. Cerutis, M.G. Nichols, S.A. Khan, T. P. McVaney, T. Miyamoto, W.B. Kahldahl. Label free imaging of the regenerating human periodontal ligament and gingival tissue using second harmonic generation confocal microscopy.  Experimental Biology 2014, April 26, 2014, San Diego, CA.

C. Miller, D. Desa, M.G. Nichols, H. Jensen Smith. Mitochondrial respiration studies suggest a novel mechanism for Aminoglycoside-induced hearing loss. 124th annual meeting of the Nebraska Academy of Sciences, April 11, 2014, Lincoln, NE.

D. Desa, C. Miller, M.G. Nichols H. Jensen Smith. Evaluating mitochondrial superoxide formation in cochlear cells during ototoxic antibiotic exposure. 124th annual meeting of the Nebraska Academy of Sciences, April 11, 2014, Lincoln, NE.

L.V. Zholudeva, K.G. Ward, M.G. Nichols, and H. Jensen-Smith. Metabolic profiling of cochlear dysfunction via two-photon fluorescence lifetime microscopy of NADH. 124th annual meeting of the Nebraska Academy of Sciences, April 11, 2014, Lincoln, NE. . (Award for Outstanding Undergraduate Research in Biomedical Sciences for 2014)

L.V. Zholudeva, M.G. Nichols, and H. Jensen-Smith. Metabolic Profiling of Cultured Murine Cochleae via Fluorescence Lifetime Microscopy.  Creighton University St. Albert’s Day/ University Research Day, April 1, 2014, Omaha, NE. (Award for Best Undergraduaate Poster Presentation)
 
A.C. Jenzer, R.C. Cerutis, M.G. Nichols, S.A. Khan, T. P. McVaney, T. Miyamoto, W.B. Kahldahl. Label free imaging of the regenerating human periodontal ligament and gingival tissue using second harmonic generation confocal microscopy.  Creighton University St. Albert’s Day/ University Research Day, April 1, 2014, Omaha, NE.

L.V. Zholudeva, K.G. Ward, M.G. Nichols, and H. Jensen-Smith. Two-Phoon Fluorescence Lifetime Imaging of Cochlear Dysfunction. Biophysical Society 58th Annual Meeting, February 16, 2014, San Francisco, CA. (Biophysical Journal 106(2) pp. 185a)

H. Jensen-Smith, D. Desa, C. Miller, M.G. Nichols.  Endogenous Differences in Cochlear Sensory and Supporting Cell Mitochondrial Metabolism Bias Free Radical Production during Ototoxin Exposure. Biophysical Society 58th Annual Meeting, February 16, 2014, San Francisco, CA (Biophysical Journal 106(2) pp. 185a)

2013

L.V. Zholudeva, M.G. Nichols, and H. Jensen-Smith. Metabolic Profiling of Cultured Murine Cochleae via Fluorescence Lifetime Microscopy. Undergraduate Poster Competition, American Society for Cell Biology Annual Meeting, December 15, 2013, New Orleans, LA

M. Lehnerz, C. Miller, D. Desa, L. Krizan, M. Barclay, L.V. Zholudeva, M.G. Nichols and H. Jensen Smith. Assessing the Impact of Antibiotics on Mitochondrial Respiration, Undergraduate Women in Physics Annual Meeting, October  24, 2013, Lincoln, NE

M. Barclay and M.G. Nichols.  Measuring Cellular Refractive Indicies with Digital Holographic Microscopy in the Near Infrared, Undergraduate Women in Physics Annual Meeting, October  24, 2013, Lincoln, NE

L.V. Zholudeva, and M.G. Nichols. Two-Photon Fluorescence Lifetime Microscopy for Profiling Cancerous Tumor Spheroids, Undergraduate Women in Physics Annual Meeting, October  24, 2013, Lincoln, NE (Best Poster Award)

L. V. Zholudeva, K.G. Ward,  M.G. Nichols and  H. Jensen-Smith. Metabolic Profiling of Cochlear Dysfunction by Fluorescence Lifetime Microscopy, Nebraska Research and Innovation Conference (NRIC): Nebraska Neuroscience Symposium, October 3, 2013, Omaha, NE

L. V. Zholudeva, K.G. Ward,  M.G. Nichols and  H. Jensen-Smith. Metabolic Profiling of Cochlear Dysfunction by Fluorescence Lifetime Microscopy, Nebraska Physiological Society Annual Meeting, October 2013, Omaha, NE

R. Fujiwara, N. Holman, M.G. Nichols, D. Sidebottom, E. J. Haas. Investigating Dynamics of Proteins in Sugar Solutions by Fluorescence Correlation Spectroscopy. Ferlic Research Presentation, September 24, 2013, Omaha, NE

L. V. Zholudeva, K.G. Ward,  M.G. Nichols and  H. Jensen-Smith. Metabolic Profiling of Cochlear Dysfunction by Fluorescence Lifetime Microscopy, Nebraska-IDeA Networks of Biomedical Research Excellence Annual Meeting, August  5, 2013, Grand Island, NE (Best Poster Award)

L. V. Zholudeva and M.G. Nichols Fluorescence Lifetime Imaging Microscopy for Profiling Cellular Metabolism in Multicell Cancer Spheroids, Creighton University Department of Chemistry Seminar, May 2013, Omaha, NE.

K.G. Ward, L. V. Zholudeva, M.G. Nichols and  H. Jensen-Smith. Let there be light! Laser scanning microscopy reveals cellular metabolism, Creighton University Alumni Meeting, April 2013, Omaha, NE

K.G. Ward and L.V. Zholudeva. Let there be light! Laser scanning microscopy reveals cellular metabolism.  17th Annual Posters on the Hill Conference, Washington, D.C., April 24 2013.

M.J. Lehnerz, D.E. Desa, L.V. Zhouldeva, M.G. Nichols. Assessing cellular metabolism by NADH FLIM and intensity-based measurements of monolayers and multicell tumor spheroids. 123rd annual meeting of the Nebraska Academy of Sciences, April 19, 2013, Lincoln, NE.

C.T. Meyer, M.G. Nichols. Design and construction of a spatially-resolved oximeter for metabolic profiling of multicell tumor spheroids. 123rd annual meeting of the Nebraska Academy of Sciences, April 19, 2013, Lincoln, NE

K. G. Ward, M.G. Nichols, H.Jensen-Smith. Ototoxic antibiotic-induced changes in cochlear metabolism generate cell-damaging free radicals. 123rd annual meeting of the Nebraska Academy of Sciences, April 19, 2013, Lincoln, NE.

L.V. Zholudeva, M.G. Nichols. Spatially resolved profiling of multicell spheroids by NADH fluorescence lifetime imaging microscopy. 123rd annual meeting of the Nebraska Academy of Sciences, April 19, 2013, Lincoln, NE.

E. Hauger, N.S. Holman, M.G. Nichols, D.L. Sidebottom, E.J. Haas. Two-photon fluorescence correlation spectroscopy of proteins in glucose solutions. 123rd annual meeting of the Nebraska Academy of Sciences, April 19, 2013, Lincoln, NE.

C.T. Meyer, M.G. Nichols. Design and construction of a spatially-resolved oximeter for metabolic profiling of multicell tumor spheroids. Creighton University St. Alberts Day/ University Research Day, March 26, 2013, Omaha, NE.

L.V. Zholudeva, M. J. Lehnerz, D. E. Desa, C. T. Meyer, M. G. Nichols. Metabolic profiling of multicell tumor spheroids by NADH fluorescence and spatially-resolved oximetry. Biophysical Society 57th Annual Meeting, February 4, 2013, Philadelphia, PA.

K. G. Ward, L.V. Zholudeva, M.G. Nichols, H. Jensen-Smith . Gentamicin Differentially Affects OHC and IHC Metabolism as Revealed by NADH Fluorescence Lifetime Imaging. Biophysical Society 57th Annual Meeting, February 4, 2013, Philadelphia, PA.

N.S. Holman, E.J. Hauger, M.G. Nichols, D.L. Sidebottom and E.J. Haas. One and Two Photon Fluorescence Correlation Spectroscopy on Proteins in Glucose Solutions. Biophysical Society 57th Annual Meeting, February 4, 2013, Philadelphia, PA.

2012

K.G. Ward. Endogenous differences in mitochondrial metabolism via ototoxic antibiotics. IDeA Network for Biomedical Research Excellence Conference in Grand Island, Nebraska. August 2012.  (Best Poster Award)

L.V. Zholudeva. Topology of Prestin. IDeA Network for Biomedical Research Excellence Conference in Grand Island, Nebraska. August 2012. (Best Oral Presentation Award)

L.V. Zholudeva, M.G. Nichols, and R. Hallworth. Two-photon fluorescence intensity and lifetime imaging of NADH reveals glucose availability in cultured cells. 122nd annual meeting of the Nebraska Academy of Sciences, April 20, 2012, Lincoln, NE.

K.G. Ward, M.G. Nichols, R. Hallworth and H. Jensen-Smith. Intensity- and lifetime-based analyses of endogenous differences in NADH metabolism in cochlear hair cells, 122 nd annual meeting of the Nebraska Academy of Sciences, April 20, 2012, Lincoln, NE.

N. Holman, Y. Wang, M.G. Nichols, and D. Sidebottom. Fluorescence correlation spectroscopy of proteins in sugar solutions 122nd annual meeting of the Nebraska Academy of Sciences, April 20, 2012, Lincoln, NE.

2011 

L.V. Zholudeva, M.G. Nichols and R. Hallworth. Two-photon fluorescence intensity and lifetime imaging microscopy of cultured cells. Conference for Undergraduate Women in Physics. Lincoln, NE. October 22nd 2011. (Best Poster Award)

K.G. Ward, H. Jensen-Smith, M.G. Nichols, R. Hallworth. Metabolic imaging of the cochlea using two-photon fluorescence imaging of NADH.  Conference for Undergraduate Women in Physics. Lincoln, NE. October 22nd 2011.

K.G. Ward. Metabolic imaging of the Cochlea. IDeA Network for Biomedical Research Excellence Conference in Grand Island, Nebraska. August 2011.

R. P. Thomen and M.G. Nichols. Single cell refractive index measurements for optical biomechanics, 121st annual meeting of the Nebraska Academy of Sciences, April 15, 2011, Lincoln, NE.

K. Ward, L. Ramsey, M.G. Nichols.  Development of a frequency-domain phase-sensitive fluorimeter to quantify the oxygen consumption of living cells. 121st annual meeting of the Nebraska Academy of Sciences, April 15, 2011, Lincoln, NE.

J. Vergen, and M.G. Nichols.  Fluorescence lifetime imaging for the assessment of cellular metabolism in multicell tumor spheroids. 121st annual meeting of the Nebraska Academy of Sciences, April 15, 2011, Lincoln, NE.

 2010

 
A. Burkart. and M.G. Nichols. Cell Stretching Determination by Static and Dynamic Optical Stretching, Department of Physics, Creighton University, Omaha. 120th annual meeting of the Nebraska Academy of Sciences, April 23, 2010, Lincoln, NE.
 
C.S. Hecht. and M.G. Nichols. Using Two-Photon Excited Fluorescence Intensity and Lifetime-Based NADH Imaging to Investigate the EMT6 Cell Line. Department of Physics, Creighton University, Omaha. 120th annual meeting of the Nebraska Academy of Sciences, April 23, 2010, Lincoln, NE.
 
R.P Thomen and M.G. Nichols. Development of a Digital Holographic Microscope, Department of Physics, Creighton University, Omaha. 120th annual meeting of the Nebraska Academy of Sciences, April 23, 2010, Lincoln, NE.
 

S. Woldemariam. and M.G. Nichols. Uncoupling Index of Refraction and Optical Path Length In Images Obtained Using a Digitial Holographic Microscope, Department of Physics, Creighton University, Omaha. 120th annual meeting of the Nebraska Academy of Sciences, April 23, 2010, Lincoln, NE.

J. Tonniges, M. Hansen, J. Duncan, M. Bassett, B. Fritzsch, B. Gray, A. Easwaran and M.G. Nichols. Diffusion Measurements of Lipophilic Fluorescence Probes in Fixed Tissue and Living Cells. Biophysical Society 54th Annual Meeting, February 20-24, 2010, San Francisco, CA.

A. Burkart, J. L. Chaput, R. P. Thomen, K.G. Ward, S.M. Woldemariam and M.G. Nichols. A Comparison of Single-cell Elasticity of Osteogenic Cells Measured with the Optical Stretcher and Holographic Microscope. Biophysical Society 54th Annual Meeting, February 20-24, 2010, San Francisco, CA.

L.M. Tiede, M.G. Nichols, R. Hallworth. Outer and Inner Hair Cells Are Metabolically Dissimilar.33rd ARO MidWinter Meeting, Anaheim, CA, February 6-10, 2010.
 
 
J. Vergen, C. Hecht, L. Tiede, and M.G. Nichols. Comparison of Two-Photon NADH Intensity Imaging and Fluorescence Lifetime Imaging To Quantify Cellular Energetics. 119th annual meeting of the Nebraska Academy of Sciences, April 17, 2009, Lincoln, NE.
 
T. Smith and M.G. Nichols. Cell Signalling and the Optical Stretcher. 119th annual meeting of the Nebraska Academy of Sciences, April 17, 2009, Lincoln, NE.
 
A. Burkart. and M.G. Nichols. Biomechanical Measurements Of Bone Cells using the Optical Stretcher., Department of Physics, Creighton University, Omaha. 119h annual meeting of the Nebraska Academy of Sciences, April 17, 2009, Lincoln, NE.
 
L. Tiede, J. Vergen, C. Hecht, R. Hallworth, M. Nichols. Using Two-photon Excited Fluorescence Intensity- and Lifetime-based NADH Imaging to Investigate Cochlea Metabolism. Biophysical Society 53rd Annual Meeting, Feb 28- March 4, 2009, Boston, MA.
 
M. Hansen, J. Tonniges, J. Duncan, M. Bassett, B. Fritzsch, B. Gray and M.G. Nichols. Diffusion-Matched and Spectrally-Discrete Lipophilic Probes for Neuronal Tracing. Biophysical Society 53rd Annual Meeting, Feb 28- March 4, 2009, Boston, MA.
 
A. Burkart, A. Lamb, M.G. Nichols. Biomechanical Measurements of Bone Cells using the Optical Stretcher. Second Annual Conference for Undergraduate Women in Physics at Yale, Yale University, New Haven, CT, January 17-18, 2009.
 

 

L.M. Tiede, A.G. Bien, P.S. Steyger, M.G. Nichols, R. Hallworth. Metabolic Imaging of the Organ of Corti – A Window On Cochlea Bioenergetics. “The Mouse As An Instrument For Ear Research III”, Jackson Laboratory, Bar Harbor, ME, September 18-21, 2008.

T.J. Smith and M.G. Nichols. Determination of Cell Elasticity in the Optical Stretcher. 7th Annual Nebraska-BRIN meeting, August 11-13, 2008, Grand Island, NE. (Third Place, poster competition)

T.J. Smith and M.G. Nichols. Determination of Cell Elasticity in the Optical Stretcher. 2nd Biennial National IDeA Symposium of Biomedical Research Excellence (NISBRE). August 6 - 9, 2008, Washington, DC. (Travel award)

C.L. Posey, A.E. Ekpenyong, M.M. Marquardt, D.L. Hames, T.J. Smith, R.P. Thomen and M.G. Nichols. Determination of Bone Cell Elasticity through Ray Optics Modeling of the Optical Stretcher. Posters on the Hill, April 30, 2008, Washington, DC.

M. Hansen, J. Duncan, B. Fritzsch, M.G. Nichols,  B. Gray,. Diffusion of fluorescently-labeled lipophilic dyes. 118th annual meeting of the Nebraska Academy of Sciences, April 18, 2008, Lincoln, NE.

J. Tonniges, M. Hansen, M.G. Nichols, B. Fritzsch, B. Gray.  Photo- and bio-physical characterization of novel blue and near-infrared lipophilic fluorophores for neuronal tracing. 118th annual meeting of the Nebraska Academy of Sciences, April 18, 2008, Lincoln, NE.

T.J. Smith and M.G. Nichols. Modeling and use of the optical stretcher and some of its potential applications for understanding changes in cell signaling due to mechanical stress. 118th annual meeting of the Nebraska Academy of Sciences, April 18, 2008, Lincoln, NE.

M.M. Marquardt, L.M. Tiede, M.G. Nichols,R. Hallworth. Two-photon excited fluorescence intensity and lifetime-based NADH imaging to determine the cellular metabolic state. 118th annual meeting of the Nebraska Academy of Sciences, April 18, 2008, Lincoln, NE.

J. Tonniges, M. Hansen, B. Fritzsch, B. Gray, M.G. Nichols. Photo- and bio-physical characterization of novel blue and near-infrared lipophilic fluorophores for neuronal tracing. Annual Meeting of the American Physical Society, March 10-14, 2008, New Orleans, LA.

C.L. Posey, A.E. Ekpenyong, M.M. Marquardt, D.L. Hames, T.J. Smith, R.K. Thomen, M.G. Nichols. Determination Cell Elasticity through Hybrid Ray Optics and Continuum Mechanics Modeling of Cell Deformation in the Optical Stretcher. 52nd Annual Meeting of the Biophysical Society, February 2–6, 2008, Long Beach, CA.

L.M. Tiede, M.M. Marquardt, M.G. Nichols,R. Hallworth. Two-photon Excited Fluorescence Intensity- and Lifetime-based NADH imaging to Determine the Metabolic Status of the Cochlea. 52nd Annual Meeting of the Biophysical Society, February 2–6, 2008, Long Beach, CA.

2007

 

C.L. Posey, A.E Ekpenyong, and M. G. Nichols. Biomechanical Measurements of Osteogenic and Non-Osteogenic  Cells using the Optical Stretcher. 6th Annual Nebraska-BRIN meeting, August 8-9, 2007, Grand Island, NE.

A.E Ekpenyong, and M. G. Nichols. Hybrid Ray Optics and Continuum Mechanics Modeling of Cell Deformation in the Optical Stretcher, Poster presented at the St. Albert’s Day session, April, 2007, Omaha, NE.

M.M. Marquardt, S.C. Hansen, and M.G. Nichols. Characterizing Changes in Cellular NADH Pools Under Various Metabolic Conditions using Two-Photon Fluorescence Lifetime Imaging. 6th Annual Nebraska-BRIN meeting, August 8-9, 2007, Grand Island, NE.

 A.E Ekpenyong and M.G. Nichols. Stress Distribution in the optical stretcher: An optimized approach for obtaining the elastic modulus of red blood cells. 117th annual meeting of the Nebraska Academy of Sciences, April 21, 2007, Lincoln, NE.

 N. Ekanayake and M.G. Nichols. Measurement of laser pulse width for Zeiss LSM 510 Meta NLO multiphoton microscope using interferometric autocorrelation. 117th annual meeting of the Nebraska Academy of Sciences, April 21, 2007, Lincoln, NE.

 V. Blake, M.R. Wyatt, and M.G. Nichols. Measurement of Clonogenic Survival of 2T3 osteoblast cells following heat shock. 117th annual meeting of the Nebraska Academy of Sciences, April 21, 2007, Lincoln, NE.

 M.M. Marquardt, R. Hallworth and M.G. Nichols. Co-localization of Prestin monomers detected by Fluorescence Resonance Energy Transfer and Fluorescence Lifetime Imaging. 117th annual meeting of the Nebraska Academy of Sciences, April 21, 2007, Lincoln, NE.

C.L. Posey and M.G. Nichols. Micromanipulation of Living Cells by the Dual-beam Optical Stretcher. 21st Annual National Conference on Undergraduate Research (NCUR), April 12-14, 2007 , Dominican University of California, San Rafael, CA.

 R.P. Wolfe and M.G. Nichols. Assessing Cell Heating during Optical Stretching using a Fluorescent Probe and Raman Spectroscopy. 21st Annual National Conference on Undergraduate Research (NCUR), April 12-14, 2007 Dominican University of California, San Rafael, CA.

M.M. Marquardt, R. Hallworth and M.G. Nichols. Co-localization of Prestin monomers detected by Fluorescence Resonance Energy Transfer and Fluorescence Lifetime Imaging. 21st Annual National Conference on Undergraduate Research (NCUR), April 12-14, 2007, Dominican University of California, San Rafael, CA.

2006

L.M. Tiede, M.G. Nichols, R. Hallworth, K. Beisel. Two-Photon, Two-Channel, Metabolic Imaging of the Organ of Corti. Optical Society of America 90th Annual Meeting, October 8 - 12, 2006, Rochester NY. Technical Digest JThC4.PDF, Conference Program pg 167.

C.L. Posey, M.M. Marquardt, R.P. Wolfe, and M.G. Nichols. Biomechanical Studies of Living Cells using the Optical Stretcher. Optical Society of America 90th Annual Meeting, October 8 - 12, 2006, Rochester NY. Technical Digest FTuE6.PDF, Conference Program pg 92.

C.L. Posey, R.P. Wolfe, and M.G. Nichols. Assessing Cell Heating During Optical Stretching. Optical Society of America 90th Annual Meeting, October 8 - 12, 2006, Rochester NY. (Undergraduate Symposium sponsored by DLS of APS). (Poster PDF 5MB )

R.P. Wolfe, C.L. Posey, and M.G. Nichols. Biomechanical Studies of Living Cells using the Optical Stretcher. Optical Society of America 90th Annual Meeting, October 8 - 12, 2006, Rochester NY. (Undergraduate Symposium sponsored by DLS of APS).

M.M. Marquardt, R. Hallworth, M.G. Nichols. FRET Between Prestin Monomers Detected by Fluorescence Lifetime Imaging. Optical Society of America 90th Annual Meeting, October 8 - 12, 2006, Rochester NY. (Undergraduate Symposium sponsored by DLS of APS).

L. Tiede, M.G. Nichols Photobleaching of reduced nicotinamide adenine dinucleotide and the development of highly fluorescent lesions in Rat Basophilic Leukemia Cells during multiphoton microscopy. Biophysical Society 50 th Annual Meeting, Feb 18-22, 2006, Salt Lake City, UT. Biophys. J. Advance Program 147A, 2006.

J. Huff, N.P. Schafer, R. Wolfe, N. Camacho, C.L.Posey, M.M.Marquardt, T. Smith, M.G.Nichols. Accessing Cell Heating During Optical Stretching by Changes in the Fluorescence Emission of Laurdan. Biophysical Society 50 th Annual Meeting, Feb 18-22, 2006, Salt Lake City, UT.Biophys. J. Advance Program 148A, 2006. (Poster PDF - 2MB)

2005

M.M. Marquardt, C.L.Posey and M.G. Nichols. Escape force calibration of the optical stretcher for biomechanics studies. 125th Annual Meeting of Sigma Xi, November 3-6, 2005 Seattle, WA. (received an award-winning superior rating by a panel of five judges)

M. Adams. Monitoring COX2 and NOS3 expression by real time RT-PCR in osteoblast-like cells deformed by an optical stretcher. 4 th Annual Nebraska-BRIN meeting, August 9-11, 2005, Grand Island, NE.

M. G. Nichols, M.A. Adams, M.M Marquardt, C.L. Posey and R. Hallworth. Development and calibration of the optical stretcher for biomechanics studies. 4th Annual Nebraska-BRIN meeting, August 9-11, 2005, Grand Island, NE.

M. Adams. Measurement of the response of osteogenic cells to mechanical stress applied by an optical stretcher. 115th annual meeting of the Nebraska Academy of Sciences, April 22, 2005, Lincoln, NE. Program Abstracts, p. 39.

J.M. Huff and M.G. Nichols. Laser induced heating in the optical stretcher. 115th Annual Meeting of the Nebraska Academy of Sciences, April 22, 2005, Lincoln, NE. Program Abstracts, p. 66.

2004

M. Adams. Measurement of the response of osteogenic cells to mechanical stress applied by an optical stretcher. 3rd Annual Nebraska-BRIN meeting, August 10-12, 2004, Grand Island, NE.

J.R. Morehead and M.G. Nichols. The optical stretcher and its application to the study of bone cells. 114th Annual Meeting of the Nebraska Academy of Sciences, April 16, 2004, Lincoln, NE. Program Abstracts, p. 48.

M. Adams and M.G. Nichols. Measurement of the response of osteogenic cells to mechanical stress applied by an optical stretcher. 114 th Annual Meeting of the Nebraska Academy of Sciences, April 16, 2004, Lincoln, NE. Program Abstracts, p. 61.

A. Gard and M.G. Nichols. Design and calibration of the optical stretcher. 114th Annual Meeting of the Nebraska Academy of Sciences, April 16, 2004, Lincoln, NE. Program Abstracts, p. 76.

2003

L. Tiede and M.G. Nichols. Photobleaching of NADH during two-photon microscopy of RBL cells. 31st Annual Meeting of the American Society for Photobiology, July 5, 2003, Baltimore, MD.

E.E. Barth, M.G. Nichols and R. Hallworth. A comparison of the sensitivity of several assays of UV-induced photodamage in rat basophilic leukemia cells. 31st annual meeting of the American Society for Photobiology, July 5, 2003, Baltimore, MD.

J.M. Reed and M.G. Nichols. Favorable conditions for mode locking a titanium sapphire laser. 113th Annual Meeting of the Nebraska Academy of Sciences, April 25, 2003, Lincoln, NE. Program Abstracts, p. 56.

J.R. Morehead, M.K. Toney and M.G. Nichols. Design and construction of a solid-state, tunable titanium: sapphire laser. 113th Annual Meeting of the Nebraska Academy of Sciences, April 25, 2003, Lincoln, NE. Program Abstracts, p. 55.

K. McGill and J. Sykora. Photon correlation spectroscopy to measure viscosity of water-glycerol mixtures. 113th Annual Meeting of the Nebraska Academy of Sciences, April 25, 2003. Lincoln, NE. Program Abstracts, p. 84.

L.M. Tiede and M.G. Nichols. NADH photobleaching during multiphoton microscopy. 113th Annual Meeting of the Nebraska Academy of Sciences, April 25, 2003, Lincoln, NE Program Abstracts, p. 55.

S. Wurdeman. Design and construction of a laser scanning confocal microscope for fluorescence- and reflectance- mode imaging of living cells. 113th Annual Meeting of the Nebraska Academy of Sciences, April 25, 2003, Lincoln, NE. Program Abstracts, p. 84.

2002

J.R. Stephenson and M.G. Nichols. Computational Modeling of the Optical Stretcher: An evaluation of the stretching force and Trap Stability for Cubic and Spherical Objects. 112 th Annual Meeting of the Nebraska Academy of Sciences, April 26, 2002, Lincoln, NE. Program Abstracts, p. 46.

J.R. Stephenson and M.G. Nichols. Size and geometry dependent trapping efficiency of the optical stretcher. 2002 April Meeting of the American Physical Society, April 11, 2002, Albuquerque, NM.

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